This paper reflects the results of research on the use of various natural biological products in the production of tomatoes in protected soil conditions. On the example of the main vegetable crops, technologies for the use of various biological products have long been developed, starting with the treatment of seeds and plants in the main phases of growth and development. In our research, we selected natural biological products recommended for use in the phases of vegetation when growing vegetable crops, including potatoes. The subjects of the biopreparations were: Abistim, Bius, Clips, Silk, and Novosil. The concentration of drugs and the treatment standards were used recommended for growing vegetable crops. It has been experimentally proven that the use of these drugs led to an increase in the number of knotted brushes and, accordingly, an increase in the fruits of commercial tomatoes was observed and, as a result, the yield per unit area increased. In the experiments, the dry matter content in the pulp and the taste qualities of the fruits increased, as well as their early ripening was observed. Thus, the tested growth regulators at all stages of development had a noticeable effect on the formation of the structure of the tomato crop. The data obtained by us confirm the fact that the sugar content in tomato fruits changes slightly during fruit ripening, and the accumulation of vitamin C increases markedly. Most vegetable crops have accumulated large reserves of ascorbic acid (vitamin C), and characteristically, this indicator determines their nutritional value. The accumulation of vitamin C goes faster when the growth processes are more intense. The tested drugs, especially Novosil and Abistim, enhance the processes of respiration and, accordingly, the growth and development of plants increases. This explains the intensive accumulation of vitamin C in tomato fruits when treated with these drugs.